Acta Univ Latv (2008) 745: 7

Orginal Article

Subcellular localization of Hepatitis B virus core protein expressed from two different constructs in the Semliki Forest virus expression system

Sabine Braun^1,2, Ruta Bruvere³, Anna Zajakina^3*, Ekaterina Alekseeva³, Tatyana Kozlovska³

¹Institut für Virologie, Charité, Humboldt-Universität, Universitätsklinikum Charité, Schumannstraße 20/21, 10117 Berlin, Germany
²Present address: Klinikum Augsburg, Institut für Laboratoriums-medizin, Mikrobiologie und Umwelthygiene, Stenglinstraße 2, 86156 Augsburg, Germany
³Department of Protein Engineering, Latvian Biomedical Research and Study Centre, Ratsupites 1, Riga LV-1067, Latvia
^*Corresponding author, E-mail: anna@biomed.lu.lv

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Abstract

The subcellular localization of Hepatitis B virus (HBV) core protein in eukaryotic cells was studied by an immunocytochemical method following expression of the HBV core (HBc) gene in the Semliki Forest virus expression system from two different constructs. HBc protein showed cytoplasmic and nuclear localization when it was expressed from a construct that encoded the HBV pregenome, whereas HBc protein that was expressed from a construct encoding the HBc gene alone was found only in the cytoplasm. The possible reasons for this phenomenon are discussed.

Key words: HBV core protein, HBV pregenome, Hepatitis B virus, Semliki Forest virus, subcellular localization.

Acta Univ Latv (2008) 745: 7–15

DOI: http://doi.org/10.22364/eeb

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Faculty of Biology, University of Latvia
				Hard copy: ISSN 1691–8088 On-line: ISSN 2255–9582 Acta Univ Latv (2008) 745: 7–15 DOI: https://doi.org/10.22364/eeb
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