Env Exp Biol (2012) 10: 125–132

Comparative genetic diversity analysis in Iranian local grapevine cultivars using ISSR and DAMD molecular markers

Abstract

The conservation and characterisation of grapevine (Vitis vinifera) genetic resources in germplasm banks have been the basis of their use in breeding programmes that result in development of new cultivars. The genetic diversity of 21 grapevine accessions from the Kurdistan province (North-west of Iran) were investigated using inter-simple sequence repeat (ISSR) and directly amplified minisatellite DNA (DAMD) markers. Average polymorphism information content for ISSR and DAMD markers was 0.43 and 0.44, respectively, which revealed the equal resolving power in both marker types. The level of polymorphism generated by ISSR markers (64%) was relatively similar to DAMD (66%) markers. Genetic dissimilarity between pairs of genotypes ranged from 0.23 to 0.81 in ISSR and from 0.12 to 0.67 in DAMD marker analysis. Cluster analysis for ISSR and DAMD markers revealed that genotypes taken for the analysis can be divided in three and two distinct clusters. Genotype clustering showed acceptable congruence between ISSR, DAMD and morphological divergence between the studied genotypes. To our knowledge, this is the first detailed report of a comparison of performance among two targeted DNA region molecular markers (ISSR and DAMD) technique on a set of samples of grapevine. Overall, our results indicate that ISSR and DAMD fingerprinting could be used to detect polymorphism for genotypes of grapevine.