Faculty of Biology, University of Latvia
Hard copy: ISSN 1691–8088
On-line: ISSN 2255–9582
Env Exp Biol (2013) 11: 119–123
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Environmental and

Env Exp Biol (2013) 11: 119–123

Original Article

Sterilization factors affect seed germination and proliferation of Achyranthes aspera cultured in vitro

Monokesh Kumer Sen*1, M. Abu Hena Mostofa Jamal1, Shamima Nasrin2
1Department of Biotechnology and Genetic Engineering, Faculty of Applied Science and Technology, Islamic University, Kushtia-7003, Bangladesh
2Plant Biotechnology Division, National Institute of Biotechnology, Savar, Dhaka, Bangladesh
*Corresponding author, E-mail: monokeshsen@yahoo.com


Achyranthes aspera (Amaranthaceae) is a highly significant medicinal herb that is common in Bangladesh. The effect of five sterilizing substances (ethanol, mercuric chloride, Flugal, Nystatin, and sodium hypochlorite) on germination and development of explants of A aspera was evaluated. The percentage of contamination and germination, as well as seed color, growth pattern, and shootlet development were determined. Among the different sterilants, sodium hypochlorite solution application for 30 min was the most effective treatment. At higher concentration, all sterilizing agents showed maximum effect against microbiological contaminantion, although the survival percentage was low.

Key words: Achyranthes aspera, microbiological contamination, seeds, sterilizing substances, tissue culture.

Env Exp Biol (2013) 11: 119–123
 DOI: http://doi.org/10.22364/eeb

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