Faculty of Biology, University of Latvia
Hard copy: ISSN 1691–8088
On-line: ISSN 2255–9582
Environ Exp Biol (2015) 13: 13–18
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Environmental and

Environ Exp Biol (2015) 13: 13–18

Orginal Articles

Isolation and identification of oil degrading bacteria from oil sludge in Abadan oil refinery

Amin Afifi1, Hossein Motamedi1,*, Bahram Alizadeh2, Hadi Leilavi1
1 Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2 Department of Geology, Faculty of Earth Science,Shahid Chamran University of Ahvaz, Ahvaz, Iran
*Corresponding author, E-mail: hhmotamedi@yahoo.com, motamedih@scu.ac.ir


Oil sludge, a rich source of hazardous pollutants that cause soil and water pollution, can be treated by biodegradation. The aim of this study was isolation of native bacteria from oil sludge produced in Abadan refinery, Iran. Native bacteria were isolated and screened based on their potential in reduction of total petroleum hydrocarbons, hemolysis, oil spreading technique and gas chromatography analysis. They were identified by 16S rRNA sequencing. Optimum N and P sources were also investigated. Pseudomonas stutzeri AOR1, through biosurfactant production, and Klebsiella pneumoniae AOR2, via degradation of long chain hydrocarbons, had the most potential in oil recovery. NaNO3 and Na2HPO4 were the optimum N and P sources for P. stutzeri, and NH4Cl and (NH4)2HPO4 for K. pneumoniae. It can be concluded that the introduced bacterial isolates have great potential in oil recovery from oil sludge, which makes them candidates for biodegradation and bioremediation purposes.

Key words: biodegradation, bioremediation, oil recovery, oil sludge.

Environ Exp Biol (2015) 13: 13–18
 DOI: http://doi.org/10.22364/eeb

Prof. Gederts Ievinsh
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University of Latvia

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