Environ Exp Biol (2015) 13: 89

Brief Communications

Sensitivity of hybrid Cymbidium to salt stress and induction of mild NaCl stress tolerance

Jaime A. Teixeira da Silva^1,2,*

¹ Faculty of Agriculture and Graduate School of Agriculture, Kagawa University, Miki-cho, Kagawa, 761-0795, Japan
² Current address: Miki-cho post office, Ikenobe 3011-2, P.O. Box 7, Kagawa-ken, 761-0799, Japan
^*Corresponding author, E-mail: jaimetex@yahoo.com

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Abstract

Research on the sensitivity of Cymbidium to NaCl does not exist. In this study, the protocorm-like bodies (PLBs) of hybrid Cymbidium Twilight Moon ‘Day Light’ were induced to form neo-PLBs on Teixeira Cymbidium (TC) medium. After initial exposure of PLBs to a range of NaCl concentrations ranging from 5 mM to 200 mM, neo-PLBs were unable to grow in the presence of NaCl that exceeded 20 mM in TC medium, showing reduced explant survival, fewer neo-PLBs per explant, and reduced fresh and dry weight. Neo-PLBs growing in medium containing 5 or 10 mM NaCl gradually adapted over several subcultures, to 40 mM NaCl in TC medium. In total, only eight neo-PLBs were found to grow on 40 mM NaCl, but none at higher levels. This simple protocol tests for NaCl stress tolerance in orchids and was found to induce mildly salt-tolerant -PLBs.

Key words: brackish water, NaCl, protocorm-like bodies, sea water, Teixeira Cymbidium medium.

Environ Exp Biol (2015) 13: 89–92

DOI: http://doi.org/10.22364/eeb

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Faculty of Biology, University of Latvia
				Hard copy: ISSN 1691–8088 On-line: ISSN 2255–9582 Environ Exp Biol (2015) 13: 89–92 DOI: https://doi.org/10.22364/eeb
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