Environ Exp Biol (2016) 14: 63–68

Geographical diversity pattern in Iranian landrace durum wheat (Triticum turgidum) accessions using start codon targeted polymorphism and conserved DNA-derived polymorphism markers

Abstract

Genetic diversity and relationships among 38 Iranian durum wheat accessions were analysed using conserved DNA-derived polymorphism (CDDP) and start codon targeted (SCoT) markers. A total of 10 CDDP and 10 SCoT primers were used to estimate genetic polymorphism among 38 durum wheat accessions. Comparatively, both CDDP and SCoT markers proved to be more effective and in terms of percentage of polymorphisms and polymorphic information content value were relatively similar. The average polymorphic information content value of CDDP was 0.39 which was relatively higher than those of SCoT where the respective values of polymorphic information content was 0.35. Using the neighbor joining clustering method, CDDP and SCoT markers were used to generate dendrograms, which revealed that the durum accessions were clustered into three and two major groups, respectively. According to the present results, CDDP markers proved more informative in studying genetic diversity among durum accessions. In both marker types, low correlation between genetic distances and geographical origin in examined durum landrace accessions was found. However, accessions from the same geographical regions mostly showed more genetic similarity than those with origins far apart. The results demostrated that CDDP and SCoT markers are useful for genetic diversity analysis of durum accessions. This information is useful for future germplasm collection and efficient utilization of landrace durum germplasm in plant breeding.