Volume 14, Number 4

Teixeira da Silva T.A., Kulus D., Zhang X., Zeng S., Ma G., Piqueras A. 2016. Disinfection of explants for saffron (Crocus sativus) tissue culture. Environmental and Experimental Biology 14: 183–198.
DOI: 10.22364/eeb.14.25

In vitro response of active vegetative buds of Crocus sativus L. after the surface disinfection of corms using the following procedure: wash in running tap water with 0.5% Cedepol, a detergent, and a few drops of Tween-20, transfer to 70% ethanol for 1 min then to 0.1% HgCl₂ for 10 min completed with five rinses in sterile distilled water. (A) Increase in corm size on 2 µM BA + 2 µM NAA + 40 to 60 g L^–1 table sugar after 12 weeks. (B) Increase in corm size on 2 µM BA + 2 µM NAA + 40 g L^–1 sucrose after 12 weeks. (C) Increase in corm size and multiple thick root formation on 8.8 µM IBA + 40 g L^–1 table sugar after 12 weeks. (D) Increase in corm size and germination on 2 µM BA + 2 µM NAA + 2.5 g L^–1 KCl + 40 g L^–1 table sugar + 30 g L^–1 sucrose after 12 weeks. Figure reproduced from Quadri et al. (2010), with kind permission from Global Science Books (www.globalsciencebooks.info).

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