Faculty of Biology, University of Latvia
Hard copy: ISSN 1691–8088
On-line: ISSN 2255–9582
Environ Exp Biol (2019) 17: 1–4
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Environmental and

Environ Exp Biol (2019) 17: 1–4

Brief Communications

The age of honey bee larvae at grafting can affect survival during larval tests

Martin Staron1, Rastislav Sabo2, Dana Staroňová1, Lucia Sabová2, Hossam F. Abou-Shaara3*
1 Research Institute for Animal Production Nitra, Institute of Apiculture Liptovský Hrádok, Slovakia
2 University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice, Slovakia
3 Department of Plant Protection, Faculty of Agriculture, Damanhour University, Damanhour, 22516, Egypt
* Corresponding author, E-mail: hossam.farag@agr.dmu.edu.eg


Honey bee larvae have been used in many experiments to assess the hazards of the environmental pollutants, including pesticides. Most studies have used one-day-old larvae to perform the experiments. This study aims to investigate the effects of larval age at grafting on survival during laboratory tests. Larvae grafted at different ages (one, three, and four days old) were compared in regard to their survival under the same laboratory conditions until hatching of adults. Our results showed that the main reason of death of larvae or pupae prior to the completion of the development was due to larval age at grafting. The grafted three-day-old larvae had better survival and were practically easier to use than the other two ages, hence the option of using larvae at this age during larval tests is supported.

Key words: honey bees, laboratory conditions, larvae.

Environ Exp Biol (2019) 17: 1–4
 DOI: http://doi.org/10.22364/eeb.17.01

Prof. Gederts Ievinsh
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University of Latvia

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