Faculty of Biology, University of Latvia
Hard copy: ISSN 1691–8088
On-line: ISSN 2255–9582
Environ Exp Biol (2019) 17: 75–83
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Environmental and

Environ Exp Biol (2019) 17: 75–83

Orginal Paper

Leaf morphological characterization and cluster analysis of Vitex negundo morphotypes

Florence Roy Salvaña1,2,3*, Kenneth Eco1, Nicci Rosietess Madarcos1, Nonnatus Bautista4
1 Graduate School, University of the Philippines Los Baños, College, Laguna, Philippines
2 Philippine Council for Agriculture, Aquatic and Natural Resources Research and Development (PCAARRD), Los Baños, Laguna, Philippines
3 Department of Biological Sciences, College of Arts and Sciences, University of Southern Mindanao, Kabacan, Cotabato
4 Plant Biology Division, Institute of Biological Sciences, College of Arts and Sciences, University of the Philippines Los Baños, College, Laguna, Philippines
* Corresponding author, E-mail: rdsalvana@usm.edu.ph


Addressing the unavailability of current knowledge associated with leaf morphological variations for Vitex negundo, this study aims to provide complete leaf characterization of 10 V. negundo morphotypes. Ten mature and fully expanded leaves were randomly collected from each standing morphotype. All collected samples were characterized and measured. A dendrogram was generated using Euclidian and Unweighted Pair Group with Arithmetic Averages analysis. Distinct variation in leaf morphology among morphotypes was observed, not typical of what is known for V. negundo. Leaves of different morphotypes primarily differ in terms of leaf shape, apex, and base. Some morphotypes also differ from others due to the unique combination of leaf types and leaf margin. The dendrogram showed two primary clusters based on leaf shape: lanceolate and non-lanceolate leaves. Other characters such as leaf type, base, apex, abaxial surface, and margin were also essential in the formation of specific clusters and separation of V. negundo morphotypes. Apparent morphological variation seen among the leaves of V. negundo accessions revealed certain characters that are atypical for a species.

Key words: dendrogram, leaf characters, morphotypes, Vitex negundo.

Environ Exp Biol (2019) 17: 75–83
 DOI: http://doi.org/10.22364/eeb.17.07

Prof. Gederts Ievinsh
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