Environ Exp Biol (2019) 17: 75–83

Leaf morphological characterization and cluster analysis of Vitex negundo morphotypes

Abstract

Addressing the unavailability of current knowledge associated with leaf morphological variations for Vitex negundo, this study aims to provide complete leaf characterization of 10 V. negundo morphotypes. Ten mature and fully expanded leaves were randomly collected from each standing morphotype. All collected samples were characterized and measured. A dendrogram was generated using Euclidian and Unweighted Pair Group with Arithmetic Averages analysis. Distinct variation in leaf morphology among morphotypes was observed, not typical of what is known for V. negundo. Leaves of different morphotypes primarily differ in terms of leaf shape, apex, and base. Some morphotypes also differ from others due to the unique combination of leaf types and leaf margin. The dendrogram showed two primary clusters based on leaf shape: lanceolate and non-lanceolate leaves. Other characters such as leaf type, base, apex, abaxial surface, and margin were also essential in the formation of specific clusters and separation of V. negundo morphotypes. Apparent morphological variation seen among the leaves of V. negundo accessions revealed certain characters that are atypical for a species.