Faculty of Biology, University of Latvia
Hard copy: ISSN 1691–8088
On-line: ISSN 2255–9582
Environ Exp Biol (2021) 19: 1–6
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Environmental and

Environ Exp Biol (2021) 19: 1–6

Orginal Paper

Protocol for micropropagation of strawberry (Fragaria × ananassa) cv. ‘Sweet Charlie’ and ‘Winter Dawn’

Madanmohan R. Dhukate1, Mafatlal M. Kher2*, A.V. Vadawale1, Priyanka Giri1
1 Plant Tissue Culture Division, GSFC Agrotech Limited (GATL), Fertilizernagar, Vadodara, Gujarat 391750, India
2 School of Science, GSFC University, Fertilizernagar, Vadodara, Gujarat 391750, India
* Corresponding author, E-mail: mafatlalmkher@gmail.com


The objective of this study was to develop a protocol for large-scale propagation of strawberry cv. ‘Sweet Charlie’ and ‘Winter Dawn’ using the micropropagation technique. Shoot cultures were obtained from shoot tips on Murashige and Skoog (MS) medium with 4% table sugar, 0.75% agar, 5 mg L−1 6-benzyladenine and 0.01 mg L−1 kinetin. These shoots were multiplied and maintained on MS medium with 1 mg L–1 6-benzyladenine and 0.1 mg L–1 kinetin. Rooting of in vitro raised shoots was successfully conducted by pulse treatment with 500 mg L–1 indole-3-butyric acid for 30 s and subsequent culturing in MS medium with 1 mg L–1 indole-3-butyric acid, 0.1 g L–1 activated charcoal and 6% table sugar. Plants were successfully acclimatized and survived in field conditions.

Key words: micropropagation, strawberry, ‘Sweet Charlie’, ‘Winter Dawn’.

Environ Exp Biol (2021) 19: 1–6
 DOI: http://doi.org/10.22364/eeb.19.01

Prof. Gederts Ievinsh
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