Faculty of Biology, University of Latvia
Hard copy: ISSN 1691–8088
On-line: ISSN 2255–9582
Environ Exp Biol (2022) 20: 55–60
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Environmental and

Environ Exp Biol (2022) 20: 55–60

Orginal Paper

Indirect organogenesis in Ephedra foliata

Mahabir Singh1, Kuldeep Yadav2, Narender Singh1*
1 Department of Botany, Kurukshetra University, Kurukshetra, India
2 Department of Botany, Gandhi Memorial National College, Ambala Cantt, Haryana, India
* Corresponding author, E-mail: nsheorankukbot11@gmail.com; mssonikuk@gmail.com


Ephedra foliata Boiss. ex C.A. Mey (Ephedraceae) is a well-known source of the alkaloid ephedrine used for the treatment of chronic asthma and associated respiratory ailments. This investigation was conducted to standardize an efficient regeneration protocol for E. foliata via indirect organogenesis through intermodal explants. Maximum callus induction (80.3%) was achieved on Murashige and Skoog (MS) medium supplemented with 0.5 mg L‑1 2,4‑dichlorophenoxyacetic acid + 0.5 mg L‑1 kinetin and additives (50 mg L‑1 ascorbic acid and 25 mg L‑1 citric acid). The maximum shoot regeneration (n = 5.27) was achieved on MS medium containing 6.0 mg L‑1 kinetin, followed by 5.0 mg L‑1 6‑benzylamino purine (n = 4.27). MS half strength medium with 3.0 mg L‑1 α‑naphthalene acetic acid resulted in the highest rooting percentage (32%). Sixty percent of the plantlets survived during acclimatization and were successfully transferred under field conditions. These plants and callus will be a suitable source of plant material for mass multiplication, genetic modification for enhanced bioactive constituents, and germplasm conservation.

Key words: callus induction, Ephedra foliata, ex vitro acclimatization, growth regulators, organogenesis.

Environ Exp Biol (2022) 20: 55–60
 DOI: http://doi.org/10.22364/eeb.20.06

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